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Direct methods for urea determination complex urea with an aldehyde or ketone under strong acidic conditions to form a red to yellow colored product, which is then measured either colorimetrically or with liquid chromatography.
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We describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia.
The determination of urea is important in a wide range of fields, including clinical diagnostics, environmental monitoring and food science.
A novel derivatization method for the determination of ethyl carbamate in spirits by liquid chromatography with spectrophotometric detection.
7 okt 2021 · This protocol measures the absorbance of urea in solution in complexation with diacetyl monoxime at 520 nm and is linearly proportional to ...
A method is described for the determination of urea in plasma and urine. The effects of variations in the experimental conditions are examined.
Abstract. Combinations of reagents are described for the catalyzed indophenol reaction for the determination of ammonia, which produces a stable blue color.
We determined the urea concentration in the serum blood by the standard Berthelot method using a diagnostic plate and a microdiagnosticum for detection in the ...
The reaction between DMAB and urea is initiated by the protonation of the dimethylamino group that produce a charge deficiency in the carbonyl carbon, making it ...
Reaction: Urease causes ammonium to be released from the urea; the ammonium can be photometrically determined according to the indophenol blue method.