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Unlabelled LRET biosensor based on double-stranded DNA for the detection of anthraquinone anticancer drugs

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Abstract

Based on upconversion nanoparticles (UCNPs) as energy donor and herring sperm DNA (hsDNA) as molecular recognition element, an unlabelled upconversion luminescence (UCL) affinity biosensor was constructed for the detection of anthraquinone (AQ) anticancer drugs in biological fluids. AQ anticancer drugs can insert into the double helix structure of hsDNA on the surface of UCNPs, thereby shortening the distance from UCNPs. Therefore, the luminescence resonance energy transfer (LRET) phenomenon is effectively triggered between UCNPs and AQ anticancer drugs. Hence, AQ anticancer drugs can be quantitatively detected according to the UCL quenching rate. The biosensor showed good sensitivity and stability for the detection of daunorubicin (DNR) and doxorubicin (ADM). For the detection of DNR, the linear range is 1–100 μg·mL−1 with a limit of detection (LOD) of 0.60 μg·mL−1, and for ADM, the linear range is 0.5–100 μg·mL−1 with a LOD of 0.38 μg·mL−1. The proposed biosensor provides a convenient method for monitoring AQ anticancer drugs in clinical biological fluids in the future.

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Funding

This research was supported in part by the Natural Science Foundation of Fujian Province (Nos. 2020J01628, 2022J011310), the Fuzhou Health Innovation Platform Construction Project (No. 2021-S-wp2), and the Fuzhou Science and Technology Plan Technology Innovation Platform Project (No. 2022-P-018).

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Authors and Affiliations

  1. Department of Orthopaedics Institute, Fuzhou Second Hospital, Fuzhou, Fujian, 350007, People’s Republic of China

    Wenhui He, Dongzhi Wu, Nannan Liu & Tao Zhang

  2. The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian, 350108, People’s Republic of China

    Zhiwei Chen, Chunxiao Yu, Yiping Shen, Xi Zhang, Fang Wu, Jinghua Chen & Jianming Lan

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  1. Wenhui He
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He, W., Chen, Z., Yu, C. et al. Unlabelled LRET biosensor based on double-stranded DNA for the detection of anthraquinone anticancer drugs. Microchim Acta 191, 15 (2024). https://doi.org/10.1007/s00604-023-06076-4

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